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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 22, Number 8
BKCSDE 22(8)
August 20, 2001 

 
Title
Effect of Alcohol Addition on Back-Extraction of BSA and Cytochrome c Using AOT Reverse Micellar System
Author
Sung-Sik Lee, Bong-Kuk Lee, Jin-Sung Choi, Jong-Pal Lee
Keywords
Back-extraction, AOT-RVMS, Percolation BSA, Cytochrome c.
Abstract
The protein back-extraction processes were discussed from the viewpoint of the micelle-micelle interaction. Bovine serum albumin (BSA) suppressing the cluster formation of reverse micelle (positive value of βpr) has the high back-extracted fraction (Eb), but cytochrome c enhancing the formation of reverse micelle (negative value of βpr) has the low back-extracted fraction, relatively. We have also examined quantitatively the effects of alcohol addition and protein solubilization on the percolation process of reverse micelle. The alcohols suppressing the formation of micellar cluster (high values of βt), remarkably improved the back-extraction rates of BSA and cytochrome c. The values of βt, defined by the variation of percolation process, and the back-extraction behavior of proteins have a good linear correlation. These results indicate that the micelle-micelle interaction or micellar clustering plays an important role in the back-extraction process of proteins.
Page
897 - 902
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