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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 23, Number 2
BKCSDE 23(2)
February 20, 2002 

Enhanced UV-Visible Absorbance Detection in Capillary Electrophoresis Using Modified T-Shaped Post-Column Flow Cell
Kwanseop Lim, Suhyeon Kim, Jong Hoon Hahn
Capillary electrophoresis, Post-column, Absorbance detection, Extended path length.
The construction of the T-shaped post-column flow cell has been changed to enhance the practicability as a UV-visible absorbance detector for capillary electrophoresis. In this new design, a rectangular cube-shaped inner structure is employed, which completely fits the outer rectangular tubing. This arrangement has greatly facilitated the fabrication of the T-cells. In addition, the volume for the auxiliary flow has been dramatically reduced down to 300 μL, and its volume flow rate is optimized at 4.2 μL/min. The short optical path length in the sheath flows (500 μm on each side) minimizes background absorption, and thus enhances its performance in low-UV wavelengths. We have optimized the auxiliary flow rate at 50 μm/s, so that migration times are insensitive to the flow rate. This optimization has improved repeatabilities in migration times and peak heights. A double-beam detection scheme using a pair of photodiodes is employed to increase the signal-to-noise ratio.
295 - 300
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