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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 23, Number 6
BKCSDE 23(6)
June 20, 2002 

 
Title
The Orientation CO in Heme Proteins Determined by Time-Resolved Mid-IR Spectroscopy: Anisotropy Correction for Finite Photolysis of Optically thick Sample
Author
Manho Lim
Keywords
Heme protein, Optically thich sample, Time-resolved IR spectroscoppy, Anisotropy.
Abstract
A systematic way of determining the equilibrium of carbon monoxide (CO) in heme proteins using time-resolved polarized mid-IR spectroscopy is presented. The polarization anosotropy at pump-probe delay time of zero inthe limit of zero photolysis and the angular distribution function of CO are required to obtain the equilibrium orientation of CO. An approach is developed for determining the polarization anisotropy in the zero-photolysis limit from the anisotropy measured under finite photolysis condition in an optically thick sample where the fraction of molecules photolyzed decreases as the pump pulse passes through and is absorbed by the sample. This approach is verified by measuring the polarization anisotropy of CO of carbonmonoxy myoglobin at various levels of photolysis. This method can be readily applied to other photoselection experiments determininf precise angle between transition dipoles.
Page
865 - 871
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