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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 25, Number 6
BKCSDE 25(6)
June 20, 2004 

 
Title
Binding Set Analysis for Interaction of Human Serum Albumin with Cethyl Trimethylammonium Bromide
Author
Abdol-Khalegh Bordbar*, Nasrin Sohrabi, Hossain Gharibi
Keywords
Binding capacity, Scatchard plot, Ionic surfactant, Human serum albumin
Abstract
The binding of cethyl trimethylammonium bromide, (CTAB) with human serum albumin (HSA) has been investigated at 5 mM phosphate buffer pH 7.0, 27 °C and various ionic strength using ion selective membrane electrodes. This method is faster and much more accurate than equilibrium dialysis technique, so provides sufficient and accurate data for binding data analysis. A novel and simple method was introduced for resolution and characterization of binding sets on basis of binding capacity concept. The values of Hill binding parameters were estimated for each set and used for calculation of intrinsic binding affinity. The results interpreted on basis of nature of forces which interfered in the interaction and represent the existence of three and two binding sets for binding of CTAB at 10?4 and 10?3 M of NaBr, respectively.
Page
791 - 795
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