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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 25, Number 6
BKCSDE 25(6)
June 20, 2004 

 
Title
Electrochemical and Spectroscopic Studies on the Interaction between DNA and the Product of Enzyme-catalyzed Reaction of OPD-H2O2-HRP
Author
S. Y. Niu, S. S. Zhang*, L. B. Ma, K. Jiao
Keywords
2,3-Diaminophenazine, DNA, Cyclic voltammetry, UV/Vis spectroscopy, Intercalation
Abstract
The pure product of 2,3-diaminophenazine was prepared by the enzyme-catalyzed reaction of ophenylenediamine-H2O2-horseradish peroxidase and characterized by UV/Vis spectroscopy, IR spectroscopy and NMR spectroscopy. The electrochemical behaviour of 2,3-diaminophenazine on the glassy carbon electrode was studied. The interaction between 2,3-diaminophenazine and deoxyribonucleic acid was studied by cyclic voltammetry method and UV/Vis spectroscopy, which indicated that the interaction between them is intercalation. The influence of reacting time was also studied. The binding ratio of the 2,3-diaminophenazine-DNA complex is calculated to be 1 : 2 and the binding constant is to be 5.07 × 103 L·mol?1 at room temperature.
Page
829 - 832
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