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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 25, Number 7
BKCSDE 25(7)
July 20, 2004 

 
Title
A New Strategy for Determining Optimum pH of Isozymes
Author
Kil-Joong Yoon
Keywords
Biosensor, Enzyme electrode, Hydrogen peroxide, Peroxidase, Isozyme
Abstract
A hydrogenperoxide sensor containing peroxidase extracted from horseradish was constructed and pH effect on its sensing ability was investigated. Current profiles of the biosensor with pH and the electrophoretic analysis showed that horseradish peroxidase consists of two isozymes. Assuming that it is a hypothetical twoisozyme mixture, the current profiles were deconvoluted into two Gaussians. Application of the new Michaelis-Menten equation connoting pH concept to this system enabled to find all the related dissociation constants of the isozyme-substrates and the isozyme-proton complexes and to determine pHs for the maximal isozyme activities.
Page
997 - 1002
Full Text
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