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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 25, Number 7
BKCSDE 25(7)
July 20, 2004 

 
Title
QCM Study of β-Casein Adsorption on the Hydrophobic Surface: Effect of Ionic Strength and Cations
Author
Myunghee Lee, Su Kyung Park, Chinkap Chung*, Hackjin Kim*
Keywords
Adsorption, β-Casein, Quartz crystal microbalance (QCM), Ionic strength, Cation
Abstract
The adsorption kinetics of β-casein on a hydrophobic surface has been studied by means of the quartz crystal microbalance (QCM). The self assembled monolayer of 1-octadecanethiol on a gold coated quartz crystal was used as a hydrophobic surface for adsorption. The adsorption kinetics was monitored in different solution conditions. Formation of monolayer is observed in most cases. At high concentration of protein, micelle formation which is interrupted by high ionic strength of solution is observed. Casein binding cations such as Ca2+, Ba2+ and Al3+ increase the hydrophobicity of the protein and the multiple layer adsorption occurs. The strong and weak points of the QCM method in the study of protein adsorption are discussed.
Page
1031 - 1035
Full Text
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