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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 25, Number 12
BKCSDE 25(12)
December 20, 2004 

 
Title
Solution State Structure of pB1, the Mimotopic Peptide of Apolipoprotein B-100, by NMR
Author
Sungran Lee, Daesung Kim, Hyojoon Kim, Yongwoo Lee, Hoshik Won*
Keywords
Apolipoprotein B-100, NMR spectroscopy, Molecular dynamic computation
Abstract
Apolipoprotein B-100 (Apo-B100) is a major protein component for low density lipoproteins (LDL). A number of mimetic peptides of Apo-B100 were screened from the phase-displayed random peptide library by utilizing monoclonal antibody (B9). Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF (pB1). From the BLAST search, the mimetic peptide pB1 had 40% homology with apo B-100. As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of 0.3-0.6 A2 and the total RMSD was 0.5-1.5 A. Moreover, pB1 structure included a weak 310-helix from Ile7 to Trp13.
Page
1845 - 1849
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