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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 25, Number 12
BKCSDE 25(12)
December 20, 2004 

 
Title
Peroxidase Activity of Cytochrome c
Author
Nam Hoon Kim, Moon Sik Jeong, Soo Young Choi, Jung Hoon Kang*
Keywords
Cytochrome c, Peroxidase, Radical scavenger, Iron chelator
Abstract
The peroxidase activity of cytochrome c was studied by using a chromogen, 2,2'-azinobis-(2-ethylbenzthiazoline-6-sulfonate) (ABTS). Initial rate of ABTS oxidation formation was linear with respect to the concentration of cytochrome c between 2.5-10 μM and H2O2 between 0.1-0.5 mM. The optimal pH for the peroxidase activity of cytochrome c was 7.0-8.5. The peroxidase activity retained about 40% of the maximum activity when exposed at 60 oC for 10 min. The peroxidase activity showed a typical Michaelis-Menten kinetics for H2O2 which Km value was 29.6 mM. Radical scavengers inhibited the peroxidase activity of cytochrome c. The peroxidase activity was significantly inhibited by the low concentration of iron chelator, deferoxamine. The results suggested that the peroxidase activity was associated with iron in the heme of cytochrome c.
Page
1889 - 1892
Full Text
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