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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 26, Number 1
BKCSDE 26(1)
January 20, 2005 

 
Title
Protein Binding Study of S-Ibuprofen Using High-Performance Frontal Analysis
Author
Longmei Jin, Du Young Choi, Haiyan Liu, Kyung Ho Row*
Keywords
S-Ibuprofen, HPFA, Binding constant, Protein
Abstract
High-performance frontal analysis (HPFA) was used for the determination of the binding constant of Sibuprofen to human serum albumin (HSA). This experiment was based on an Inertsil 100 Diol 5 column and sodium phosphate buffer (pH 7.4 and ionic strength of 0.17) as the mobile phase. The mixture of S-ibuprofen and HSA (70 μM) solution were directly injected into the HPFA column. An injection volume of 200 μL and a “restricted injection” method were applied to ensure the drug to be eluted as a zonal peak with a plateau. The unbound drug concentration was calculated from the peak height of the zonal peak. Scatchard analysis was used for evaluation of the binding constant (K) and binding affinity (nK) of S-ibuprofen to HSA, and the results were K = 2.833 × 104 [L mol.1], nK = 4.935 × 104 [L mol.1], respectively.
Page
136 - 138
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