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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 26, Number 3
BKCSDE 26(3)
March 20, 2005 

 
Title
Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip
Author
Yong-Sung Choi*, Kyung-Sup Lee, Dae-Hee Park
Keywords
Indicator-free DNA chip, Immobilization, Target DNA, Hybridization, Ferricyanide
Abstract
This research aims to develop DNA chip array without an indicator. We fabricated microelectrode array by photolithography technology. Several DNA probes were immobilized on an electrode. Then, indicator-free target DNA was hybridized by an electrical force and measured electrochemically. Cyclic-voltammograms (CVs) showed a difference between DNA probe and mismatched DNA in an anodic peak. Immobilization of probe DNA and hybridization of target DNA could be confirmed by fluorescent. This indicator-free DNA chip microarray resulted in the sequence-specific detection of the target DNA quantitatively ranging from 10-18 M to 10-5 M in the buffer solution. This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.
Page
379 - 383
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