Current time in Korea 03:27 Oct 19 (Thu) Year 2017 KCS KCS Publications
KCS Publications
My Journal Log In Register
HOME > Search > Browsing(BKCS) > Archives

Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 26, Number 10
BKCSDE 26(10)
October 20, 2005 

 
Title
Simultaneous Quantitative Determination of Monosaccharides Including Fructose in Hydrolysates of Yogurt and Orange Juice Products by Derivatization of Monosaccharides with p-Aminobenzoic Acid Ethyl Ester Followed by HPLC
Author
Joung Ho Ko, Huazi Huang, Gyoung Won Kang, Won Jo Cheong*
Keywords
Monosaccharides, Reductive amination, p-Aminobenzoic acid ethyl ester, HPLC
Abstract
We have determined the contents of monosaccharides in the hydrolysates of some yogurt and orange juice products by derivatizing monosaccharides with p-aminobenzoic acid ethyl ester (ABEE). The separation of the ABEE-derivatized monosaccharides was efficiently carried out by HPLC using a microcolumn packed with the Alltima C18 stationary phase. The concentrations of monosaccharides were determined based on the measured peak area/height counts. ABEE derivatization of fructose and its detection have never been successfully carried out before this work. In this study, two peaks were observed in a fixed ratio for ABEE-fructose, and the ratio was maintained over a wide range of fructose concentration. In order to prove the validity of the above method, we compared the concentrations of glucose, galactose and fructose determined by ABEE derivatization and UVD (ultraviolet detector) chromatography with those determined by RID (refractive index detector) chromatography without derivatization. The determined concentrations of monosaccharides obtained from the two chromatographic methods were found close to each other within acceptable error ranges.
Page
1533 - 1538
Full Text
PDF