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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 27, Number 10
BKCSDE 27(10)
October 20, 2006 

 
Title
[Ru(phen)2(dppz)]2+ Assemble on the Surface of the SDS Micelle and Its Application for the Determination of DNA
Author
Fang Chen*, Jianping Huang, Zhike He
Keywords
[Ru(phen)2(dppz)]2+, SDS, DNA, Resonance light scattering
Abstract
The solution of [Ru(phen)2(dppz)]2+ and SDS has high Resonance Light Scattering (RLS) signals due to [Ru(phen)2(dppz)]2+ assemble on the surface of the SDS micelle. Because of the high affinity (KB ≥ 106 L mol-1) between [Ru(phen)2(dppz)]2+ and DNA, the adding of DNA in the solution of [Ru(phen)2(dppz)]2+-SDS makes the dissociation of [Ru(phen)2(dppz)]2+-SDS, and results in decreasing of the RLS signals and increasing of the absorbance. Based on this, a novel method is proposed for DNA assay. Under optimum condition, good linear relationship was obtained within the concentration range of 0.018-1.26 μg mL?1, the linear equation is IRLS = 504.8-348.8 c (c: μg mL-1) and the correlation coefficient (r) is 0.9992. The detect limit for calf thymus DNA is 8.6 ng mL-1. The proposed method was successful applied to determine the extracted colibacillus plasmid DNA.
Page
1655 - 1658
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