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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 29, Number 1
BKCSDE 29(1)
January 20, 2008 

 
Title
Binding Affinities of Carbohydrate-Conjugated Chlorins for Galectin-3
Author
Young K. Park, Bayarmaa Bold, Bing Cun Cui, Jin Quan Bai, Woo-kyoung Lee, Young Key Shim*
Keywords
Biosensor, Cancer marker, Carbohydrate, Chlorin, Galectins
Abstract
Carbohydrate-conjugated chlorins were synthesized for use as biosensors for the detection of the galectin-3 cancer marker. We used ELISA, SDS-gel electrophoresis, and Bradford assays to examine the binding of galectins to D-(+)-galactose- and β-lactose-conjugated chlorins. The binding affinities of these conjugated chlorins for galectin-3 were quantified using fluorescence spectroscopy. The fluorescence emission of the carbohydrate-conjugated chlorins decreased as the amount of galectin-3 in the binding reaction increased over a limited concentration range, indicating that carbohydrate-conjugated chlorins are potentially useful fluorescence biosensors for the galectin-3 cancer marker.
Page
130 - 0
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