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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 29, Number 10
BKCSDE 29(10)
October 20, 2008 

 
Title
Mg2+-dependency of the Helical Conformation of the P1 Duplex of
the Tetrahymena Group I Ribozyme
Author
Joon Hwa Lee
Keywords
NMR, Mg2+, RNA, Imino proton exchange, Group I ribozyme
Abstract
The P1 duplex of Tetrahymena group I ribozyme is the important system for studying the conformational changes in folding of ribozyme. The formation of the P1 duplex between IGS and substrate RNA and the catalytic activity of ribozyme require a variety of metal ions such as Mg2+ and Mn2+. In order to investigate the effect of the Mg2+ concentration on the conformation of the P1 duplex, the NMR study was performed as a function of Mg2+ concentration. This study revealed that the less stable AU-rich region formed duplex at 50 oC under high Mg2+ concentration condition but melts out under low Mg2+ concentration condition. It was also found that in the active conformation under 10 mM MgCl2 condition, the unstable central G·U wobble pair maintains the significant base pairing up to 50 oC. This study provides the information of the unique feature of the P1 duplex structure and the roll of Mg2+ ion on the formation of the active conformation.
Page
1937 - 1940
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