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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 29, Number 11
BKCSDE 29(11)
November 20, 2008 

Analysis of Benzoic Acid in Quasi-Drug Drink Using Isotope Dilution Liquid Chromatography Mass Spectrometry
Insun Lee, Seonghee Ahn*, Byungjoo Kim, Eujin Hwang, Yong Seong Kim
Isotope dilution liquid chromatography mass spectrometry (ID-LC/MS), LC/tandem MS, Uncertainty
An analytical method was developed for the accurate determination of benzoic acid in commercial quasi-drug drinks using isotope dilution liquid chromatography mass spectrometry (ID-LC/MS). Sample drinks were spiked with 13C6-benzoic acid and these mixtures were cleaned by a nylon membrane filter. The sample mixtures were then analyzed using LC/Tandem MS in the negative ion mode without further clean-up or extraction steps. For the chromatographic separation, a C18 column with isocratic mobile phase of 50:50 (v/v) ammonium acetate buffer at pH 4.5 and methanol mixture was used. MS was operated with electrospray ionization in the negative mode. Benzoic acid and its isotope analogue were detected by selectively monitoring the collisionally-induced dissociation channels of [M-H]? → [M-CO2H]? at m/z 121 → 77 and m/z 127 → 83, respectively. Repeatability and reproducibility studies showed that the ID-LC/MS/MS method is a reliable and reproducible method which can provide less than 0.3% of relative standard deviation (RSTD) of method precision for analysis of benzoic acid.
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