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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 29, Number 12
BKCSDE 29(12)
December 20, 2008 

 
Title
Differential Epitope-Mapping of the Two Forms of the Prion Protein: Alterations at the C-Terminus
Author
Seong Soo A An*, Janet Barletta, Neil Constantine
Keywords
Prion, Epitope, Conformation specific antibody
Abstract
Most of the diagnostic methods for transmissible spongiform encephalopathies are based on the detection of the abnormal protease-resistant conformers of the prion protein (PrPSc), when the normal protease-sensitive conformers (PrPC) are also present. A selective immune detection of PrPSc with high sensitivity is hampered by the absence of sufficient knowledge about the structure of PrPSc. An economical and rapid mapping approach is used here for the identification of epitopes that are exposed differently on PrPSc and PrPC. This approach revealed remarkable differences in the conformation/accessibility of the two long helical segments between the two prion forms. This result is not in harmony with recent models of PrPSc.
Page
2403 - 2406
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