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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 31, Number 6
BKCSDE 31(6)
June 20, 2010 

NTA·Ni2+-Functionalized Quantum Dots for VAMP2 Labeling in Live Cells
Mi Kyung Yu, Suho Lee, Sunghoe Chang*, Sangyong Jon*
Quantum dot conjugates, Imaging probes, Labeling, Protein tracking
An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag (His6-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) (NTA·Ni2+) on the QD surfaces. We showed that the NTA·Ni2+-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted His6-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.
1474 - 1478
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