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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 31, Number 6
BKCSDE 31(6)
June 20, 2010 

 
Title
Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles
Author
Yeon Ji Lee, Jong Moon Park, Jiyoung Huh, Minsik Kim, Je Sun Lee, Arudra Palani, Kwangyeol Lee*, Sang Won Lee*
Keywords
Proteomics, Glutathione, Superparamagnetic, Nanoparticle, Separation
Abstract
The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.
Page
1568 - 1572
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