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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 3
BKCSDE 32(3)
March 20, 2011 

 
Title
Selective 3,4-Dihydroxyphenylalanine Analysis in Human Urine as Ethoxycarbonyl-tert- butyldimethylsilyl Derivatives by Gas Chromatography-Mass Spectrometry
Author
Man Jeong Paik, Duc Toan Nguyen, Jaehwan Yoon, In Seon Cho, Wooyoung Shim, Kyoung Rae Kim, Ki Hong Cho, Sangdun Choi, Gwang Lee*
Keywords
3,4-Dihydroxyphenylalanine (DOPA), Ethoxycarbonyl/tert-butyldimethylsilyl derivatives, Gas chromatography-mass spectrometry, Urine sample
Abstract
A new analytical method for measurement of 3,4-dihydroxyphenylalanine (DOPA) in human urine was developed. DOPA from an aqueous solution was converted into an ethoxycarbonyl (EOC) derivative. A tert-butyldimethylsilyl (TBDMS) reaction under anhydrous conditions was then attempted for analysis by gas chromatography-mass spectrometry in selected ion monitoring mode. A new mass spectral data on DOPA as a tri-EOC/mono-TBDMS derivative was built. This method showed good linearity (r ≥ 0.999), precision (% relative standard deviation = 3.1-9.2), and accuracy (% relative error = −7.2-8.8), with a detection limit of 0.05ng/mL. This selective and accurate method of DOPA analysis will be useful for biochemical monitoring of various neurological disorders including Parkinson’s disease in biological fluids.
Page
977 - 980
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