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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 4
BKCSDE 32(4)
April 20, 2011 

 
Title
A Simple and Sensitive High Performance Liquid Chromatography-Electrospray Ionization/Mass Spectrometry Method for the Quantification of Ethyl Pyruvate in Rat Plasmac
Author
Hyun Ji Kim, Seung Woo Kim, Ja Kyeong Lee, Sung Hwa Yoon*
Keywords
Ethyl pyruvate, Quantification, Liquid chromatography-mass spectrometry, Electrospray ionization, Sodium adduct formation
Abstract
Ethyl pyruvate (EP) is known as a scavenger of reactive oxygen species (ROS) in the body through its role in the donation of diketone groups to metals to form an EP-metal complex. In order to develop a method for the quantification of EP in biological media, a sensitive and specific, high-performance liquid chromatographyelectrospray ionization-mass spectrometry (HPLC-ESI/MS) method is used to determine the EP-alkali metal ion binding species. The analyte was separated on a ZORBOX SB-C8 (3.5 μm, 30 mm × 2.1 mm I.D.) column and analyzed in selected ion monitoring (SIM) mode with a positive ESI interface using the m/z 255 [2M + Na]+ ion. The method was validated over the concentration range of 0.5-60.0 μg/mL under 1/9 (v/v) of acetonitrile/methanol solvent system with flow rate 0.05 mL/min. The limit of quantification (LOQ) was 0.5 μg/mL.
Page
1221 - 1227
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