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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 7
BKCSDE 32(7)
July 20, 2011 

 
Title
Synthesis and structural studies of a pharmaceutical salt and its association with BSA
Author
Fa-Yan Meng, Sheng-Rong Yu, Li-Xi Liang, Xue-Ping Zhong, Li Wang, Jin-Mei Zhu, Cui-Wu Lin *
Keywords
Bovine serum albumin, Organic complex, Chlorogenic acid, Fluorescence quenching, 2,2'- Bipyridine
Abstract
The self-assembly of one novel organic complex based on chlorogenic acid (HCA) and 2,2'-bipyridine (2,2'- bipy) has been synthesized and characterized. The complex achieved by hydrogen-bonding interactions, adopted a 1:1 stoichiometry in a solid state. The proton transfer occurred from the carboxyl oxygen to the aromatic nitrogen atom to form salts CA·(2,2'-Hbipy), the 2,2'-Hbipy molecule individually occupies the pseudo-tetragonum that is formed with CA. In this paper, the interactions of CA·(2,2'-Hbipy) with bovine serum albumin (BSA) were studied by fluorescence spectrometry. For CA·(2,2'-Hbipy), HCA and 2,2'-bipy, the average quenching constants for BSA were 2.4384 × 104, 4.653 × 103, and 3.059 × 103 L·mol−1, respectively. The mechanism for protein fluorescence quenching is apparently governed by a static quenching process. The Stern-Volmer quenching constants and corresponding thermodynamic parameters ΔH, ΔG and ΔS were calculated. The binding constants and the number of binding sites were also investigated. The conformational changes of BSA were observed from synchronous fluorescence spectra.
Page
2253 - 2259
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