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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 32, Number 9
BKCSDE 32(9)
September 20, 2011 

 
Title
Interaction of Bilobalide and Ginkgolides B with Bovine Serum Albumin: A Fluorescence Quenching Study
Author
Yan Chen, Ruijun Wang*, Shusheng Wang, Yi Yang, Shaofei Li, Guiqing Kai
Keywords
Bilobalide, Ginkgolides B, Bovine serum albumin, Fluorescence spectroscopy, Interaction
Abstract
The interaction of bilobalide (BB) and ginkgolides B (GB) with bovine serum albumin (BSA) was investigated by fluorescent technique and UV/vis absorption spectroscopy. The results showed that BB and GB could intensively quench the fluorescence of BSA through a static quenching procedure. The binding constants (Ka) and the average binding distance between the donor (BSA) and the acceptor (ginkgolides) were obtained (rBB = 5.33 nm and rGB = 4.20 nm) by the theory of non-radiation energy transfer, and then the thermodynamic parameters such as ΔS0 (0.17-0.32 kJ/mol), ΔG0 (-20.76 ~ -17.79 kJ/mol) and ΔH0 (32.47-76.52 kJ/mol) could be calculated, respectively. All these results revealed that the interaction of BB and GB with BSA were driven mainly by hydrophobie force. The synchronous fluorescence spectroscopy was applied to examine the effect of two ginkgolides on the configuration of BSA. The configuration alteration of BSA could be induced by the hydrophobicitv environment of tyrosine with the increase of the drug concentration.
Page
3261 - 3266
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