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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 33, Number 1
BKCSDE 33(1)
January 20, 2012 

 
Title
A New Chemiluminescence Method for Determination of Cytosine Arabinoside in Pharmaceutical Preparations
Author
Z. Cai, * X. Zhang, D. F. Lu, J. N. Gan
Keywords
Flow injection, Chemiluminescence, Eosin Y, Cytosine arabinoside, Hydroxyl radical
Abstract
A novel chemiluminescence (CL) system was established for the determination of cytosine arabinoside (Ara- C) in pharmaceutical preparations. It was showed that a clear CL signal was observed when Eosin Y mixed with Fenton reagent. The CL intensity was decreased significantly when Ara-C was added to the reaction system and partially scavenged the hydroxyl radicals in the solution. The extent of decrease in the CL intensity had a good stoichiometrical relationship with the Ara-C concentration. Based on this, we developed a new method for the determination of Ara-C using a flow injection analysis (FIA) technique with CL detection. Under the optimal conditions, the linear range of Ara-C concentration was 6.0 × 10−9 ~ 1.0 × 10−7 mol/L (R = 0.9982) with a detection limit of 7.6 × 10−10 mol/L (S/N=3) , the RSD was 5.6% for 6.0 × 10−8 mol/L Ara- C (n = 11). The method was successfully applied to the determination of Ara-C in injection samples. The possible chemiluminescence reaction mechanism was discussed.
Page
171 - 176
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