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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 33, Number 2
BKCSDE 33(2)
February 20, 2012 

 
Title
Determination of Coccidiostats (Amprolium and Decoquinate) in Cattle and Chicken's Muscle using High Performance Liquid Chromatography
Author
ByungJu Kim, Hyunsun Ham, Jin-Joo Lee, Nam-Yong Cheong, Seung-Woon Myung*,
Keywords
Coccidiostats, Amprolium, Decoquinate, HPLC, Simultaneous determination
Abstract
An analytical method for the simultaneous determination of veterinary medicines (amprolium and decoquinate) in cattle and chicken's muscle by HPLC/UV-vis was established. Samples were extracted by a HLB (Hydrophilic-Liphophilic Balance) cartridge with acetonitrile and methanol. Prior to HPLC injection, a mixture solvent (Water:MeOH, 1:1) was utilized as a reconstitution solvent. Chromatographic separation was achieved with a C18 column (250 × 4.6 mm, 5 μm) using gradient elution with 20 mM HFBA and MeOH:ACN (1:1.8). The calibration curves from the spiked blank matrix showed good linearity (above r2=0.997) in the concentration range of 0.13-12.0 mg kg−1. The relative recovery (accuracy) and limit of quantitation (LOQ) were in the range of 78.5-107.1% and 0.13-0.42 mg kg−1, respectively. The developed method can be used to determine under the MRL (Maximum Residue Limits) levels of veterinary medicines in animal tissues.
Page
559 - 563
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