Current time in Korea 01:57 Oct 18 (Wed) Year 2017 KCS KCS Publications
KCS Publications
My Journal Log In Register
HOME > Search > Browsing(BKCS) > Archives

Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 33, Number 7
BKCSDE 33(7)
July 20, 2012 

 
Title
Sample Preparation and Stability of Human Serum and Urine Based on HPLC-DAD for Metabonomics Studies
Author
Yun Liu, Xiaoming Sun, Duolong Di*, Yuxiang Feng, Fengling Jin
Keywords
Stability, HPLC/UPLC, Serum, Urine, Metabonomics
Abstract
Many literatures focus on the biological relevance and the identification of biomarkers for disease activity assessment while less attention has been paid to the development of standard procedures for sample preparation and storage based on liquid chromatography technique. The influencing factors including protein precipitation, storage temperature, storage time, and reconstitution by ultra pure water were analyzed employing HPLCDAD. The effects were investigated from five participants over three months by principal components analysis (PCA) and the values of percent changes (PC). The samples with protein precipitation might slow the rate of bacterial enzymatic conversion. After protein precipitation, the average PC of urine samples (0.136 ± 0.013, n = 5) is relatively less than that of the serum samples (0.173 ± 0.026, n = 5) for three months. Minimal effects on metabolic profiles of serum and urine (PC < 0.15) are reasonable for metabolomic studies after protein precipitation and storage at −20 oC for two months.
Page
2156 - 2162
Full Text
PDF