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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 34, Number 3
BKCSDE 34(3)
March 20, 2013 

Effect of Number and Location of Amine Groups on the Thermodynamic Parameters on the Acridine Derivatives to DNA
Ji Hye Kwon, Hee-Jin Park, Nataraj Chitrapriya, Sung Wook Han, Gil Jun Lee*, Dong Jin Lee, Tae-Sub Cho*
9-Aminoacridine, Proflavine, Intercalation, Themodynamics
The thermodynamic parameters for the intercalative interaction of structurally related well known intercalators, 9-aminoacridine (9AA) and proflavine (PF) were determined by means of fluorescence quenching study. The fluorescence intensity of 9AA decreased upon intercalation to DNA, poly[d(A-T)2] and poly[d(G-C)2]. A van’t Hoff plot was constructed from the temperature-dependence of slope of the ratio of the fluorophore in the absence and presence of a quencher molecule with respect to the quencher concentration, which is known as a Stern-Volmer plot. Consequently, the thermodynamic parameters, enthalpy and entropy change, for complex formation was calculated from the slope and y-intercept of the van’t Hoff plot. The detailed thermodynamic profile has been elucidated the exothermic nature of complex formation. The complex formation of 9AA with DNA, poly[d(A-T)2] and poly[d(G-C)2] was energetically favorable with a similar negative Gibb’s free energy. On the other hand, the entropy change appeared to be unfavorable for 9AA-poly[d(G-C)2] complex formation, which was in contrast to that observed with native DNA and poly[d(A-T)2] cases. The equilibrium constant for the intercalation of PF to poly[d(G-C)2] was larger than that to DNA, and was the largest among sets tested despite the most unfavorable entropy change, which was compensated for by the largest favorable enthalpy. The favorable hydrogen bond contribution to the formation of the complexes was revealed from the analyzed thermodynamic data.
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