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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 34, Number 3
BKCSDE 34(3)
March 20, 2013 

 
Title
Chemical Imaging Analysis of the Micropatterns of Proteins and Cells Using Cluster Ion Beam-based Time-of-Flight Secondary Ion Mass Spectrometry and Principal Component Analysis
Author
Hyun Kyong Shon, Jin Gyeong Son, Kyung-Bok Lee, Jinmo Kim, Myung Soo Kim, Insung S. Choi*, Tae Geol Lee*
Keywords
Chemical imaging, Protein and cell micropatterns, ToF-SIMS, PCA
Abstract
Micropatterns of streptavidin and human epidermal carcinoma A431 cells were successfully imaged, as received and without any labeling, using cluster Au3 + ion beam-based time-of-flight secondary ion mass spectrometry (TOF-SIMS) together with a principal component analysis (PCA). Three different analysis ion beams (Ga+, Au+ and Au3 +) were compared to obtain label-free TOF-SIMS chemical images of micropatterns of streptavidin, which were subsequently used for generating cell patterns. The image of the total positive ions obtained by the Au3 + primary ion beam corresponded to the actual image of micropatterns of streptavidin, whereas the total positive-ion images by Ga+ or Au+ primary ion beams did not. A PCA of the TOF-SIMS spectra was initially performed to identify characteristic secondary ions of streptavidin. Chemical images of each characteristic ion were reconstructed from the raw data and used in the second PCA run, which resulted in a contrasted – and corrected – image of the micropatterns of streptavidin by the Ga+ and Au+ ion beams. The findings herein suggest that using cluster-ion analysis beams and multivariate data analysis for TOF-SIMS chemical imaging would be an effectual method for producing label-free chemical images of micropatterns of biomolecules, including proteins and cells.
Page
815 - 819
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