Current time in Korea 19:18 Oct 21 (Sat) Year 2017 KCS KCS Publications
KCS Publications
My Journal Log In Register
HOME > Search > Browsing(BKCS) > Archives

Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 34, Number 4
BKCSDE 34(4)
April 20, 2013 

 
Title
Specific Isoforms of Protein Kinase G Downregulate the Transcription of Cyclin D1 in NIH3T3
Author
Seon Young Lim, Jae-Won Soh*
Keywords
PKG, Cyclin D1, c-fos, NF-κB, CRE
Abstract
To elucidate the role of PKG isoforms in transcriptional control of cyclin D1, we employed a series of expression vectors of PKG 1α and PKG 1β which encode HA-tagged wild type and constitutively active (SD and ΔN) mutants. Our present study demonstrates that both the constitutively active mutants of PKG 1β downregulate the transcription of cyclin D1 when transiently transfected in NIH3T3 cells, whereas PKG 1α mutants show weak inhibition. We further studied the transcriptional regulators of cyclin D1, such as, c-fos, NF-κB, and CRE by using the luciferase reporter assay. Constitutively active mutants of PKG 1β showed marked transcriptional downregulation of c-fos in NIH3T3 cells, whereas PKG 1α downregulated c-fos to a lesser extent. We also found that the constitutively active mutants of PKG negatively regulated the activation of NF-κB and CRE, suggesting their involvement in the regulation of cyclin D1.
Page
1165 - 1169
Full Text
PDF