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Bulletin of the Korean Chemical Society (BKCS)

ISSN 0253-2964(Print)
ISSN 1229-5949(Online)
Volume 35, Number 9
BKCSDE 35(9)
September 20, 2014 

 
Title
Synchrotron-based Transmission X-ray Microscopy (TXM) Observations of Fully Hydrated Blood Platelets and Their Activation Process
Author
Nuri Yang, Hyun Woo Nho, Yogesh Kalegowda, Jin Bae Kim, Jaewoo Song, Hyun-Joon Shin, Tae Hyun Yoon*
Keywords
Transmission X-ray microscopy, Whole mount electron microscopy, Platelets, Platelet activation, In situ microscopy
Abstract
Platelets are anuclear discoid-shaped blood cells with key roles in human body. To understand the mechanisms of their activation process, it is required to have analytical imaging techniques capable of acquiring platelet images under fully hydrated conditions. Herein, for the first time, we demonstrate the capability of synchrotronbased transmission X-ray microscopy (TXM) to study platelets (resting and ADP activated) under hydrated and air-dried conditions. To confirm the biological imaging capability of TXM, fixed platelets were imaged and compared with whole mount electron microscopy (EM) images. TXM provided morphological information with sufficient spatial resolution with simple and quick sample preparation procedure. We also observed temporal changes during the platelet activation, which initially had a discoid shape (0 s), formed pseudopodia (30 s) and generated a network of fibrin (5 min). Our results clearly demonstrate the potential of TXM technique to study fully hydrated biological samples under in situ conditions.
Page
2625 - 2629
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